Sean Foxley1, Vandana Sampathkumar1, Vincent De Andrade2, Scott Trinkle1, Anstasia Sorokina1, Katrina Norwood1, Patrick La Riviere1, and Narayanan Kasthuri1
1University of Chicago, Chicago, IL, United States, 2Advanced Photon Source, Argonne National Laboratory, Lamont, IL, United States
1University of Chicago, Chicago, IL, United States, 2Advanced Photon Source, Argonne National Laboratory, Lamont, IL, United States
A single postmortem mouse brain was imaged with MRI, mCT, and electron microscopy, with resolution scales spanning 5 orders of magnitude. This imaging pipeline gives us an unprecedented and contextually seamless view across the multi-scaled organization of the brain.
FIGURE 4: (a) 3D rendering of structural MRI data used for coregistration with (Fig 3a) µCT volumetric data. Somas and dendrites of neurons in the medial vestibular nucleus (e) and somas in (g) dentate gyrus were traced from the µCT data. While these somas are much smaller than the resolution of the MRI data, they provide possible underlying sources of contrast in the T2* weighted images.
FIGURE 2: The same brain was imaged using (a, b) MRI (50 mm isotropic voxels), (c, d) µCT (1.2 mm isotropic voxels), and (e, f) EM (3 nm in-plane resolution). Panels (b) and (c) show the same FOV from the MRI and µCT imaging (the red ROI in (a)). Panels (d) and (e) show magnified µCT and EM data (the green ROI in (c)). Yellow arrows indicate corresponding blood vessels, and a single neuron is colored purple. Panel (f) shows an individual somatic synapse (white arrows) on that soma, colored orange (the blue ROI in (e)).
