Jessie Mosso1,2,3, Julien Valette4, Katarzyna Pierzchala1,2, Dunja Simicic1,2,3, Ileana Ozana Jelescu1,2, and Cristina Cudalbu1,2
1CIBM Center for Biomedical Imaging, Lausanne, Switzerland, 2Animal Imaging and Technology, EPFL, Lausanne, Switzerland, 3LIFMET, EPFL, Lausanne, Switzerland, 4Commissariat à l'Energie Atomique (CEA), Institut d'Imagerie Biomédicale (I2BM), Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses, France
1CIBM Center for Biomedical Imaging, Lausanne, Switzerland, 2Animal Imaging and Technology, EPFL, Lausanne, Switzerland, 3LIFMET, EPFL, Lausanne, Switzerland, 4Commissariat à l'Energie Atomique (CEA), Institut d'Imagerie Biomédicale (I2BM), Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses, France
A
significant decrease in the diffusion coefficient of Gln and an increase for
Tau and Glu in the cerebellum of a rat model of chronic hepatic encephalopathy
was measured, reflecting changes in astrocytes morphology measured ex vivo by histology.
A. Representative neurometabolic profiles
in BDL and Sham rats acquired at 14.1T in the cerebellum (STEAM sequence -
TE=3ms, TM=10ms, LB=8Hz) B. Representative spectra at different b-values
for BDL and SHAM rats acquired with the STE-LASER4 sequence
(LB=8Hz) in the cerebellum at 14.1T. C. Individual metabolite decays versus b-values
for both groups. Error bars: dispersion around the mean value. Statistics: 2-way
ANOVA with variables disease and b-value, *: p<0.05.
IHC staining in the cerebellum of Sham and BDL rats. Top:
anti-GFAP(red)/DAPI(blue) staining of the cerebellar folium, scale bar: 100μm.
Middle: GFAP/DAPI staining of the granular layer, scale bar: 25μm. Bottom:
Colgi-Cox staining of Purkinje cells, scale bar: 25μm Right: corresponding
quantifications, ** p<0.01, *** p<0.001, **** p<0.0001.
