Kumar Pichumani1, Omkar Ijare1, Elizabeth Maher2, Robert M Bachoo2, and David S Baskin1
1Peak Center, Neurosurgery, Houston Methodist Hospital, Houston, TX, United States, 2UT Southwestern Medical Center, Dallas, TX, United States
1Peak Center, Neurosurgery, Houston Methodist Hospital, Houston, TX, United States, 2UT Southwestern Medical Center, Dallas, TX, United States
Pyruvate recycling is active in primary GBM and metastatic brain tumors. Intravenously infused 13C labeled glucose during the surgery is used to track the flux through this pathway in patients using resected tumor tissues.
Schematic diagram illustrating pyruvate recycling pathway during [U-13C]glucose metabolism.
C2 lactate 13C NMR spectral region of brain tumor tissue extracts. The patients were given intravenous infusion of [U-13C]glucose prior to
the surgical removal of the tumor. D12 and D23 doublets (via pyruvate recycling) represent [1,2-13C] and [2,3-13C]lactate isotopomers. The
quartet (Q) signals correspond to the [U-13C]lactate that are generated from glycolysis of [U-13C]glucose.