Multi-Frequency Magnetization Transfer (MFMT) Sequence design. MFMT is a saturation prep followed by 3d-FLASH acquisition. MFMT saturation prep allows multiplexing up to four different/independent offset frequencies each with independent bandwidths – thereby simultaneously saturating up to four different frequencies. Sat RF pulses are Gaussian and run with RF phase stepping (RF spoiling).

Representative result Hippocampal transverse plane images qualitatively show a clear increase in contrast between MFMT ‘On’ versus ‘Off’. The yellow arrows specifically point to hippocampal regions and details with obvious contrast increase when MFMT is ‘On’.