Byeong-Yeul Lee1, Jeffrey M. Solomon2, Marcelo Castro1, Dong-Yun Kim3, Joseph Laux1, Matthew G. Lackemeyer1, Jordan K. Bohannon4, Anna N. Hanko5, Dima Hammoud6,7, and Ji Hyun Lee1
1Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD, United States, 2Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, United States, 3Office of Biostatistics Research, National Heart, Lung and Blood Institute, Bethesda, MD, United States, 4National Biodefense Analysis and Countermeasures Center, Frederick, MD, United States, 5Microbiology, Boston University School of Medicine, Boston, MA, United States, 6Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, United States, 7Center for Infectious Disease Imaging, National Institutes of Health, Bethesda, MD, United States
1Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD, United States, 2Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, United States, 3Office of Biostatistics Research, National Heart, Lung and Blood Institute, Bethesda, MD, United States, 4National Biodefense Analysis and Countermeasures Center, Frederick, MD, United States, 5Microbiology, Boston University School of Medicine, Boston, MA, United States, 6Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, United States, 7Center for Infectious Disease Imaging, National Institutes of Health, Bethesda, MD, United States
In this work, we found significant increases in T1 and R2* values in the multiple brain regions in a nonhuman primate model of aerosolized Ebola virus (EBOV) infection, including the cerebellum and occipital lobe, which provides in vivo evidence of brain involvement with EBOV.
Figure 1. Averaged T1 map (n=7) of the rhesus macaque brain before and
after EBOV exposure: Compared to pre-exposure (top row), the post-exposure T1
map (bottom row) showed noticeable increases in multiple regions, including cerebellum, occipital lobes, deep gray matter, and frontal cortices (white
arrows and dotted circles).
Figure
2. A voxel-based statistical comparison of T1 of the rhesus macaque brain
before and after EBOV exposure: There were significant increases in T1 values in
various brain regions, in particular in the cerebellum and occipital lobes. Statistical
significance was considered at corrected p < 0.001 (one-tailed
paired t-test, false discovery rate correction). The colorbar shows a T-score range.
