Martina Vermathen1, Tobias Emmanuel Kämpfer1,2, and Peter Vermathen2
1Department of Chemistry and Biochemistry, University Bern, Bern, Switzerland, 2Departments of BioMedical Research and Radiology, University Bern, Bern, Switzerland
1Department of Chemistry and Biochemistry, University Bern, Bern, Switzerland, 2Departments of BioMedical Research and Radiology, University Bern, Bern, Switzerland
The photosensitizer chlorin e4 induces already in the dark a dose
dependent metabolic response in cells that is attenuated by PVP and
block-copolymer micelle carriers shown by HR-MAS NMR. Direct interaction of Ce4
with cellular GPC was detected indicating membrane localization.
FIGURE 2
PCA applied to all samples and buckets from 1D projected spectra (259 x 39) (A). Excerpts from the PCA plot in (A) for better visibility of the subclasses, i.e. samples without carriers (B), samples with KP as carrier (C) and samples with PVP as carrier (D).
FIGURE 5
(A) HR-MAS 1H-diffusion-edited NMR spectra (1D-DOSY) of lysed HeLa cell suspensions in PBS incubated with different concentrations of Ce4 without carrier or encapsulated into KP or PVP. Shown is the spectral region where the choline containing resonances appear. The GPC resonance exhibits upfield shifts;
(B) Chemical shift of the GPC-choline resonance as function of condition (Ce4 concentration and carrier)
