Characterization of 3AM diffusion MRI phantoms via microscopy, and phase-contrast micro-CT
Farah Mushtaha1,2, Tristan K. Kuehn1,3, Omar El-Deeb4, Seyed A. Rohani3, Luke W. Helpard3, Hanif Ladak2,3,5, Amanda Moehring6, Corey A. Baron1,2,3,7, and Ali R. Khan1,2,3,7
1Robarts Research Institute, London, ON, Canada, 2Medical Biophysics, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, 3School of Biomedical Engineering, Western University, London, ON, Canada, 4Neuroscience, Western University, London, ON, Canada, 5Department of Electrical and Computer Engineering, Western University, London, ON, Canada, 6Biology, Western University, London, ON, Canada, 7The Brain and Mind Institute, Western University, London, ON, Canada
Pore diameters of the 3AM phantoms measured using microscopy and micro-CT were in agreement with a mean pore diameter of ~ 8 μm, and fits of both kurtosis and ball and stick models were in agreement between simulation and acquired dMRI data.
Figure 3. Synchrotron micro-CT scan data at two zoom levels, transformed to align lines of material with the viewing planes. Upper row: View of the entire scan ROI. Lower row: Detailed view of a short length of five stacked lines of material. The direction of print-head motion was left-right in the left- and right-most columns, and perpendicular to the image in the center column.
Figure 1. a) Confocal microscopy z-stack image of a stained phantom slide. Elastomeric matrix (red) and pores (black) are visible. Each white outline indicates an individual line of material. b) 2D projection of a 3D microscopy volume acquired with confocal microscopy. Outlined in yellow are larger pores caused by the printing pattern of the phantom. In both a and b, the image plane is perpendicular to the long axis of the pores.