Athanasia Kaika1, Geoffrey J. Topping1, Mathias Schillmaier1, and Franz Schilling1
1Department of Nuclear Medicine, Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Munich, Germany
1Department of Nuclear Medicine, Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Munich, Germany
Filter-exchange spectroscopy (FEXSY) measures cell-membrane
water permeability changes (confirmed by trypan blue staining) caused by isopropanol,
TritonX-100 and ultrasonic treatment, or water flux through the cell-membrane due
to osmotic imbalance.
Figure 3: a) FEXSY signal before and after cell wash. The spectrum peaks were used
for the calculation of the ADCs for water and isopropanol, and AXR for water. (b,
c) AXR
relaxation curves fits for cell pellets before (b) and after the cell wash (c) for
several isopropanol treatment intervals. (d, e, f, g) FEXSY measurements of the cell pellet before (yellow, red)
and after the cell wash (blue) over time. (e, f, g) ADC values calculated from
the FEXSY signal with diffusion filter off.
Figure 4: (a) AXR relaxation
curves with AXR fit of the control and two yeast cell suspensions treated with 0.4% Triton X-100
concentrations for 15
minutes and 12.50h. The AXR curves were calculated from the water
peak of the FEXSY signal spectrum. (b) Percentage of trypan blue stained
cells of each suspension.
