Alexander James Wilson1, Kevin M Moulin2, Gregory B Sands3, and Daniel B Ennis2
1Radiology, Stanford University, Palo Alto, CA, United States, 2Radiology, Stanford University, Stanford, CA, United States, 3Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand
1Radiology, Stanford University, Palo Alto, CA, United States, 2Radiology, Stanford University, Stanford, CA, United States, 3Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand
A physics-based diffusion tensor MRI simulation of a confocal tissue volume yielded a transmural
helix angle well matched to structure tensor analysis. Direct comparisons of confocal
tissue volumes with cardiac DTI are feasible and can provide insight to
experimental design.
Figure
1: Overview of study design. (Left) Flow chart of the main study steps
from imaging, through segmentation and analysis to comparison of results. (Top Right) Histology image produced from the
extended volume confocal microscopy, presented using a ‘Glow’ look up
table. (Bottom right) The same image after
segmentation of the following compartments: intracellular (blue), extracellular
(red) and blood vessel/cleavage space (green).
Figure 3: Results of the diffusion tensor
simulation. Vector representations of the primary
eigenvector of the diffusion tensor analysis from the four rows of voxel-blocks
(top row and middle row). Color maps of
the helix angle (bottom). The vector
plots show a transition from longitudinal myofibers at the epicardium, through
circumferential fibers at the mid-wall, to longitudinal fibers at the
endocardium.