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Marlena Rohm^1,2, Gabriele Russo^3,4, Xavier Helluy^3,5, Martijn Froeling⁶, Denise Manahan-Vaughan³, Matthias Vorgerd^1,2, and Lara Schlaffke^1
1Department of Neurology, BG-University Hospital Bergmannsheil gGmbH, Bochum, Germany, ²BG-University Hospital Bergmannsheil gGmbH, Heimer Institute for Muscle Research, Bochum, Germany, ³Department of Neurophysiology, Ruhr-University Bochum, Bochum, Germany, ⁴International Graduate School of Neuroscience, Ruhr-University Bochum, Bochum, Germany, ⁵Department of Biopsychology, Institute of Cognitive Neuroscience, Faculty of Psychology, Ruhr-University Bochum, Bochum, Germany, ⁶Department of Radiology, University Medical Centre Utrecht, Utrecht, Netherlands

Keywords: Muscle, Translational Studies, Longitudinal Studies

In Pompe disease a mutation in the alpha-glucosidase gene leads to accumulation of glycogen and autophagosomes. Previously, we reported changes in diffusion in patient muscles. To identify histopathological correlations, the quantitative MRI protocol was translated to a mouse model, using DTI sequences in a monthly interval to scan the hind limb of pre-symptomatic mice. Subsequently, immunofluorescence stainings and glycogen assay were carried out to correlate glycogen accumulation and autophagic buildup with diffusion changes. No fat-infiltration was detected, while FA increases significantly compared to wildtype. Changes in diffusion parameters were correlated to autophagic buildup but did not correlate with glycogen accumulations.

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Jialin Wang¹, Xin Mu², Mingyan Wu¹, Yanbin Li¹, Huahui Xu^3,4, Xiaoli Gu^3,4, and Jeff L. Zhang^2
1Central Research Institute, United Imaging Healthcare, Shanghai, China, ²School of Biomedical Engineering, ShanghaiTech University, Shanghai, China, ³Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, China, ⁴Department of Radiology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai, China

Keywords: Skeletal, Elastography

Using magnetic resonance elastography (MRE), we investigated the potential changes in the stiffness of calf muscle with plantar flexion. Significant decreases in shear modulus were found when exercise was applied in a progressive way. Such decrease was possibly due to the increase muscle hyperemia in exercise, which was partially verified by increased T2-weighted signals in the same study. In conclusion, MRE is a promising method for measuring mechanical viscoelastic properties of skeletal muscles and thus for assessing muscle function or performance.

Shan Lu¹, Shiyu Zhao¹, Xueyan Song¹, Guangyang Ma¹, Zhiwei Shen², and Baocheng Chang*^1
1Metabolic Diseases Hospital of Tianjin Medical University, Tianjin, China, ²Philips Healthcare, Beijing, China

Keywords: Muscle, Infection

 For the first time, DF infection was analyzed using ASL and APTw imaging, and high BF and MTRasym (3.5 ppm) may be used as a novel biomarker in DF course monitoring and treatment option evaluation. ASL and APTw imaging in DF may be a safe and convenient approach for individuals with renal insufficiency because to its non-contrast injection.